INTENDED USE
Rapid Heartworw/Ehrlichia/Lyme/Anaplasma Combo test kit is a sandwich lateral flow immunochromatographic assay for the qualitative detection of Heartworm Ag, Ehrlichia Ab, Lyme Ab and Anaplasma Ab in dog’s blood.
PRINCIPLE
Rapid Heartworw/Ehrlichia/Lyme/Anaplasma Combo test kit is based on sandwich lateral flow immunochromatographic assay. The test device has a testing window. The testing window has an invisible T (test) zone and C (control) zone. When sample is applied into the sample hole on the device, the liquid will laterally flow on the surface of the test strip. If there is enough antigen/antibody in the sample, a visible T band will appear. The C band should always appear after a sample is applied, indicating a valid result. By this means, the device can accurately indicate the presence of antigen/antibody in the sample.
KIT COMPONENT
-Test device, with disposable dropper
-Assay buffer
-Products Manual
TEST PROCEDURE
1. Collect dog’s whole blood and centrifuge it to get serum or plasma. Or use whole blood with anticoagulants as specimen. For whole blood sample without anticoagulant must be tested immediately.
2. Specimens and test kit stored at 2-8℃ should be recovered to room temperature before use.
3. Take out the test device from the foil pouch and place it horizontally.
4. Using dropper add 2 drops of Heartworm serum to the sample hole. Other 3 samples use dropper to dispense 10ul (black line) specimen to the sample hole. Then drip 3 drops of buffer into each sample hold.
5. Interpret the result in 5-10 minutes. Result after 10 minutes is considered as invalid.
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